Mispa-i2 is a semi-automated specific protein analyzer that offers high precision and quick turnaround results, for all protein assays in blood testing and supports the clinical management of variety of diseases such as Diabetes, Cardiovascular risk, Inflammation, etc. Mispa i2 has a patented Unique Channel Shifting (UCS) technology for best sensitivity and high linearity which ensures quality results.

What is protein analysis and how can it be done with a protein analyzer?

Proteins are large, complex molecules that play many critical roles in the body. Proteins differ from each other in their size, molecular structure, and physiochemical properties. These differences allow for protein analysis, and it is the standard method of protein analysis to determine protein concentration.

The large-scale study of proteins is called Proteomics. Protein quantification is necessary to understand the protein content in a sample and further prognosis. A protein analyzer is a handy laboratory tool for determining the protein quantity in a sample.

An analytical chemistry technique called nephelometry is used to quantify how cloudy or turbid a solution is as a result of suspended insoluble particles. Light is transmitted, absorbed (blocked), and dispersed as it passes through a turbid solution with suspended solid particles. The size, shape, and concentration of the insoluble particles in solution as well as the wavelength of the input light all affect how much light is scattered. In the nephelometry technique the amount of this scattered light is measured with the help of a nephelometry detector placed at a specific angle (usually at 90 degree). Nephelometry was originally used in immunoassays for the detection and measurement of serum proteins in the blood, such as immunoglobulins and macromolecules, in the field of clinical chemistry.

The amount of light that passes through the sample is measured by photometry. In photometry, a sample containing insoluble particles in solution is illuminated with light of a specified wavelength, and the light that passes through the sample is collected by a detector positioned in line with the light source.

The concentration of the sample and the size of the scattering particles are the two key determinants of whether to use nephelometry or photometry. The intensity of the transmitted light will be about equivalent to the intensity of the light source if the sample includes few insoluble particles (low concentrations). Nephelometry is a better option in this situation. Since light transmission is greatly reduced in samples with a high concentration of insoluble particles, photometry is a better fit.

Difference between conventional nephelometer and new generation nephelometer

Conventional nephelometers works based on endpoint nephelometry and new generation nephelometers work based on rate-based or kinetic nephelometry.

Endpoint (or fixed-time) nephelometry detects the maximum scattered light after an antigen-antibody interaction has achieved equilibrium, or after a defined reaction time. Rate or kinetic nephelometry is an alternate approach that uses repeated observations over time used over the whole precipitation process and often offers more information on the reaction than endpoint nephelometry method.

Product description and an overview of Mispa-i2 specific protein analyzer

For all protein tests in blood testing, the Mispa-i2 is a semi-automated specialized protein analyzer that provides excellent accuracy and rapid turnaround times. Mispa i2 helps the clinical treatment of a range of disorders like Diabetes, Cardiovascular risk, Inflammation, etc. by providing a broad test panel of 25 parameters.

The product Mispa i2 is entirely produced in India and aids in quantitative specific protein quantification by combining the industry-recognized nephelometry and turbidimetry approaches. The Mispa i2 uses a special, patented technology to deliver the highest sensitivity and linearity, ensuring high-quality findings.

A decade ago, nearly 35 percent of specific protein estimations were done by the latex method. The latex methods are qualitative/semi-quantitative tests. Due to this limiting factor of latex testing, there was always an uncertainty of results in the borderline areas of reference ranges. These tests were affordable to the rural population but due to its low specificity and sensitivity, the purpose of the testing was eluded in many cases.

Approximately 45 percent of the protein estimations were performed in turbidimetry assays in fully automated or semi-automated systems at a higher cost. Nephelometry, the best method for protein testing, was unreachable to the common laboratories because of the very high cost of the automated instruments, reagents, and consumables.

With the motive to provide high-quality protein estimation at an affordable cost, Agappe introduced this flagship product for protein estimation in 2012. Within a decade, Mispa i2 emerged as the game changer in specific protein estimation.

How does Mispa -i2 differ from other protein analyzers?

The dual measuring technology (rate-based nephelometry and immunoturbidimetry) with the unique patented technology - UCS (Unique Channel Shifting) makes Mispa i2 different from other conventional nephelometers.

Mispa i2 comes with smart card calibration for all parameters, thus saving time and cost. Also, the rate-based nephelometry technique in Mispa i2 helps in reducing false reporting through the prozone detection feature.

Working principles of Mispa-i2 specific protein analyzer

Mispa i2 works based on the combination of two methodologies - one is rate-based nephelometry, and the other is immunoturbidimetry. Hence the analyzer has 2 detectors namely, the nephelometry detector and a turbidimetry detector.

Rate based nephelometry method offers high sensitivity and the immunoturbidimetry method offers high linearity. Thus, the dual measuring technique offers a wide measuring range and ensures accurate results at all protein concentrations.

Mispa i2 serves 25 parameters with better sensitivity and linearity which ensures flexibility in laboratory testing with marginal investment. Mispa i2 offers reliable results with a convenient pack size for all 25 parameters. The assay panel includes cardiovascular risk profile, allergy profile, covid profile, diabetic profile, rheumatology profile, osteoporosis screen profile, etc. Major parameters include HbA1c, CRP, RF, ASO, D Dimer, Ferritin, Microalbumin, immunoglobulins, Cystatin C, C3, C4, etc.

Each reagent pack of Mispa i2 comes with a smart card which contains lot-specific calibration data. Mispa i2 offers automated smart card calibration. The smart card calibration feature omits the need for a lengthy calibration process and reduces manual calibration errors. It also saves calibration costs, and reagent costs, and saves time. Hence this feature is quite beneficial for parameters like HbA1c, CRP, RF, etc. which require multipoint calibration.

The rate-based nephelometry technique used in Mispa i2 helps in prozone detection and thus reduces false reporting chances. Mispa i2 reads the rate of absorbance continuously, and any repetition of similar absorbance at two different time instants is equivalent to the excess of antigen or antibody in the reaction cuvette (prozone or Kendall effect).

Benefits of the Specific Protien Analyzer : Mispai2

Mispa i2 works based on Rate Based Nephelometry & Immuno-turbidimetry technology. Rate Based Nephelometry and Immunoturbidimetry are analytical chemistry methods for determining the amount of cloudiness, or turbidity in a solution during the blood testing.

The technology is based upon the measurement of the effect of turbidity upon the transmission and scattering of light through the sample. In Nephelometry the intensity of the scattered light is measured, whereas in Turbidimetry the intensity of light transmitted through the sample is measured, often at a particular angle. The combination of two measuring technologies ensures high linearity and sensitivity.